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KMID : 0377619760310010033
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Korean Jungang Medical Journal
1976 Volume.31 No. 1 p.33 ~ p.48
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Nucleic Acid Contents and Guanine Anzinohydrolase Activity and Histopathological- studies in Auxiliary Liver Grafts Supplied by Arterial Blood alone in Rats
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羅成均/Rha, Sung-Kyun
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Abstract
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In order to asses that the hepatic arterial blood alone can maintain the function of the heterotopic auxiliary liver graft in rats, guanine aminohydrolase (GDA) GPT, and GOT activities as well as nucleic acids were, analyzed in the graft.
Isogenie and allogeneic liver transplantation were performed on Lewis and Sprague-Dawley rats respectively, weighing 250 to 300 gm by the suture technique of Lee´s original method, but only arterial blood flow was introduced to the graft and the bile duct was implanted into the duodenum.
All´hepatic isografts in these experiments were removed 1, 4, 5, 6,10, and 13
(days subsequent to the surgical ,procedure for biochemical studies, and for histo￢pathological studies-in the grafts allogeneic liver transplant rats were also sacrificed for periods up to 14 days.
The following results were obtained.
I .Biochemical studies
1), DNA. contents were decreased, with slight increases on the 5th postoperative day.
2) RNA contents showed also decreases, except slight increases on the 5th postoperative day.
3) GDA activities were also decreased, maximally oil the 5th postoperative day, but progressive increases were evident on the 13th postoperative day. 4) ,GPT & GOT activities showed marked decreases.
Histopaaholoical studies:
n observation period the graft shod-ed to
slight increase on the DLIi postopu ative day
2), Some of the grafts revealed findings of rejection, outflow blurb, in flamma Lion and/or infection, or combined features of them.
It is concluded that the. hepatic arterial blood can maintain minimal liver fu￢nction, but have no apparant regeneration,power, in the isogeneic liver graft. The histopathological study in the allogenic liver graft discloses the findings, of rejection, outflow,block, inflammation, ischemia and combined features of them.
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